Unit of Competency Mapping – Information for Teachers/Assessors – Information for Learners

MSL974025 Mapping and Delivery Guide
Prepare tissue and cell cultures

Version 1.0
Issue Date: May 2024


Qualification -
Unit of Competency MSL974025 - Prepare tissue and cell cultures
Description
Employability Skills
Learning Outcomes and Application This unit of competency describes the skills and knowledge to prepare primary tissue cultures for applications, such as maintenance of animal cell lines and propagation of plants by tissue culture, and basic subculture procedures. Personnel are required to manipulate equipment and materials and samples to prevent contamination at all preparation stages. They will have ready access to workplace procedures and will work under direct supervision.This unit applies to technical assistants working in laboratories in the biomedical, environmental, biotechnology and education industry sectors.No licensing or certification requirements exist at the time of publication. However, regulations and/or external accreditation requirements for laboratory operations exist, so local requirements should be checked. Relevant legislation, industry standards and codes of practice within Australia must also be applied.
Duration and Setting X weeks, nominally xx hours, delivered in a classroom/online/blended learning setting.
Prerequisites/co-requisites
Competency Field Testing
Development and validation strategy and guide for assessors and learners Student Learning Resources Handouts
Activities 		Slides
PPT 		Assessment 1 Assessment 2 Assessment 3 Assessment 4
Elements of Competency Performance Criteria              
Element: Maintain records
  • Maintain records of batches of media
  • Complete records of tissue cultures so that they are retrievable, legible and accurate
  • Complete records in accordance with information management, records, quality system and legal requirements
       
Element: Work safely according to the legal and regulatory framework
       
Element: Identify hazards and workplace controls associated with the sample, preparation methods, reagents and equipment
       
Element: Select, fit and use personal protective equipment (PPE)
       
Element: Address hazards and incidents as they arise
       
Element: Maintain a chain of custody, traceable to the worker, for all cells and tissues
       
Element: Prepare and test cell and tissue culture media
  • Select and confirm media specifications and processes/methods
       
Element: Prepare culture media to suit the application
       
Element: Sterilise culture media and check for sterility
       
Element: Perform quality control checks to ensure that culture media is fit for purpose
       
Element: Store culture media in accordance with specifications
       
Element: Prepare tissue or cell cultures
  • Select tissue/cell sample to optimise growth and prepare it for culture
       
Element: Add specified growth agents and/or nutrients
       
Element: Inoculate culture medium using aseptic techniques
       
Element: Monitor tissue or cell culture
  • Incubate culture in specified conditions
       
Element: Monitor growth of culture and record appearance and characteristics
       
Element: Report presence or absence of contamination
       
Element: Subculture the culture to continue the cell line
       
Element: Dispose of biohazardous and other laboratory waste safely
  • Maintain records of batches of media
       
Element: Complete records of tissue cultures so that they are retrievable, legible and accurate
       
Element: Complete records in accordance with information management, records, quality system and legal requirements
       

Submission Requirements

List each assessment task's title, type (eg project, observation/demonstration, essay, assignment, checklist) and due date here

Assessment task 1: [title]      Due date:

(add new lines for each of the assessment tasks)

Assessment Tasks

Copy and paste from the following data to produce each assessment task. Write these in plain English and spell out how, when and where the task is to be carried out, under what conditions, and what resources are needed. Include guidelines about how well the candidate has to perform a task for it to be judged satisfactory.
Copy and paste from the following performance criteria to create an observation checklist for each task. When you have finished writing your assessment tool every one of these must have been addressed, preferably several times in a variety of contexts. To ensure this occurs download the assessment matrix for the unit; enter each assessment task as a column header and place check marks against each performance criteria that task addresses.

Observation Checklist

Tasks to be observed according to workplace/college/TAFE policy and procedures, relevant legislation and Codes of Practice Yes No Comments/feedback
Maintain records of batches of media 
Complete records of tissue cultures so that they are retrievable, legible and accurate 
Complete records in accordance with information management, records, quality system and legal requirements 
 
 
 
 
 
Select and confirm media specifications and processes/methods 
 
 
 
 
Select tissue/cell sample to optimise growth and prepare it for culture 
 
 
Incubate culture in specified conditions 
 
 
 
Maintain records of batches of media 
 
 

Forms

Assessment Cover Sheet

MSL974025 - Prepare tissue and cell cultures
Assessment task 1: [title]

Student name:

Student ID:

I declare that the assessment tasks submitted for this unit are my own work.

Student signature:

Result: Competent Not yet competent

Feedback to student

 

 

 

 

 

 

 

 

Assessor name:

Signature:

Date:

Assessment Record Sheet

MSL974025 - Prepare tissue and cell cultures

Student name:

Student ID:

Assessment task 1: [title] Result: Competent Not yet competent

(add lines for each task)

Feedback to student:

 

 

 

 

 

 

 

 

Overall assessment result: Competent Not yet competent

Assessor name:

Signature:

Date:

Student signature:

Date: